Effect Of Different Storage Conditions Research Articles

Effect of different storage conditions on grain quality of paddy

The present investigation was carried out to study the physiological and biochemical changes of paddy grain (Oryza sativa L. var. Ranjit) throughout the course of a 12-month storage period, in an effort to determine the ideal storage conditions with the highest potential for storage under climacteric condition of Assam. Freshly harvested seeds of paddy grain (Ranjit) were stored under different storage conditions for a period of twelve months beginning in January. Based on the results of the study, it was found that the easily moisture permeable structures (T2, T3, T6) showed the maximum change in the physiological and biochemical parameters followed by permeable (T1 and T4) and impermeable (T5 and T7). The rate of change in the values of parameters in paddy stored in the prototype of a Grain safe™ in-store dryer (T7) was found slowest and sluggish, and maintained highest mean values amongst all the storage receptacles in respect of germination percentage, seed vigour index, head rice recovery, moisture content, total soluble protein content, starch content, total soluble sugar content, reducing sugar, non-reducing sugar and amylose content. Significant superiority of hermetic storages (T5 and T7) over traditional storage structures (T1, T2, T3, T4 and T6) in Assam irrespective of all the parameters studied was noticed. Hermetic storage was effective at blocking the effects of external humidity fluctuations as well as the spread of fungi and insects. The solar in-store drier was found to be best amongst all storage structures, in all quality aspects of the long-term storage of paddy.

Comparative study on the impact of storage conditions on ESR signals in fingernail dosimetry

Human fingernails in combination with electron spin/paramagnetic resonance (ESR/EPR; hereafter “ESR”) spectroscopy have high potential as an individual dosimetry tool in radiological accidents. For the practical realization of this technique, it is necessary to reduce the uncertainty due to the instability of ESR signals affected by both individual and environmental factors. In the present study, we examined the effects of different storage conditions (in a laboratory room, refrigerator, freezer, and vacuum) and prior sample treatment on the intensity and stability of ESR signals from the fingernails of a child, which has not been investigated in previous studies, in comparison with those of an adult. Fingernail samples collected from a 10 y/o female child and a 37 y/o male adult were irradiated with 160 kV X-rays at 10 Gy, and the time changes of radiation-induced ESR signal (RIS) and background signal (BKG) under different conditions were measured for up to 30 days. As results, the ESR signal intensities (peak-to-peak amplitudes) of both child and adult fingernails were the most stable under freezing conditions, while the intensities of both RIS and BKG of fingernails were notably higher and more stable in the child samples than in the adult samples. In addition, the child samples showed a better response to the prior treatment using ethanol and water; BKG faded significantly after treatment despite the original higher background. Further investigations with more samples from donors of different ages are needed to clarify and reduce inter- and intra-individual variability in the dosimetric properties of human fingernails.

Storage Conditions Influence the Quality of Ginger - A Stability Study Inspired by Clinical Trials.

Ginger has traditionally been used to treat and prevent nausea and vomiting; however, the results of clinical trials are ambiguous. The efficacy of ginger is attributed to gingerols and their metabolites, shogaols. Since these compounds have different pharmacological profiles, the clinical efficacy of ginger products is largely dependent on their chemical composition. The goal of our study was to examine the stability of ginger determining the 6-gingerol contents in order to assess the effects of different storage conditions. We have performed a 6-month stability test with dry ginger rhizome samples stored in a constant climate chamber in three different storage containers (uncovered glass container, glass container sealed with rubber stopper, plastic container). 6-gingerol contents were measured by HPLC method. The concentration of 6-gingerol decreased in all samples. In the sealed glass container, the decrease of 6-gingerol content was significantly lower than in the unsealed glass container and in the plastic container. These results demonstrate that storage conditions have a significant impact on the quality of ginger, which may also affect efficacy.

The Effects of Different Storage Conditions on Leukocytes in Human Breast Milk.

Breast milk is the best baby food because it contains various nutrients and important factors for the baby's immune system, including leukocytes. This study aimed to determine the effects on morphology, number of cells and breast milk leukocytes count of various ways of storing breast milk based on different temperatures and storage durations. This study was conducted at the Biochemistry Laboratory, Integrated Laboratory and the Histology Laboratory, Universitas Indonesia, Jakarta, Indonesia from September 2022 to February 2023. Transitional breast milk samples from 7 breastfeeding mothers were utilised in the study. A total of 50 mL was divided into 4 tubes of 12.5 mL each and treated based on temperature, storage time and method of thawing frozen breast milk based on the Centers for Disease Control and Prevention's (CDC) recommendations for breast milk storage. The breast milk cells were isolated to calculate the cell number and leukocyte population. Subsequently, the breast milk cells were stained with haematoxylin and eosin to analyse the number and morphology of leukocytes. The findings showed a significant decrease in the breast milk's total number and population and changes in the morphology of breast milk leukocytes after storage. This study indicates that CDC storage recommendations do not affect the quantity of the CD45+ leukocyte population; however, there is a decrease in the total number of leukocytes and alterations in their microscopic morphology. Thus, additional research is recommended to determine whether these modifications influence the function of the breast milk cells.

Effect of different storage conditions on dimensional accuracy of 3D-printed dental models.

The objective was to determine the accuracy of 3D-printed dental models subjected to different storage conditions using six different material and printer combinations. Three completely dentate models were designed using dental CAD software (3Shape Dental System). A horseshoe-shaped solid base with a posterior horizontal bar was used. The models were printed in a horizontal direction against the building platform without support. The models were printed using six printers with the corresponding recommended resin material: Carbon M2 (DPR10), HeyGears A2D4K (Model HP UV2.0), Stratasys J5 (MED610), Stratasys Origin One (DM200), Envision One (E-Model LightDLP), and Asiga Pro4K (VeriModel) with a standard layer thickness of 50 μm. All printed models underwent scanning using a laboratory scanner (Sirona inEOS X5) after printing. Subsequently, the models were randomly assigned into three groups of storage conditions, LT: cold environment (4±1°C), HT: hot and dry environment (50±2°C), and RT: room temperature (25±2°C) serving as the control. Each group was kept under the designated condition and was scanned at 1, 2, 3, 4, and 8 weeks. The total number of models (N) was 72, with 6 printers producing 12 models per printer for 3 storage conditions, resulting in 4 models for each storage condition and each printer. The generated STL files were imported into a 3D inspection software for comparison with the original STL files. In-tolerance percentage, the deviation RMS, trueness, and precision were obtained and analyzed with least square mean linear regression using JMP Pro 15 to identify the significant effects (α = 0.05). The in-tolerance percentage as-printed was significantly different among different printers. Significant dimension deviations were observed after the first week of storage at HT and with subsequent weeks of storage. RT and LT did not show significant dimensional changes. Models printed with Carbon M2 showed the highest in-tolerance percentages compared to the other printers. The model deviations were affected by storage conditions and the printer used, with high-temperature storage showed least stability compared to low and room temperatures. No significant difference was observed between low and room temperature storage conditions. The Carbon M2 printer showed the highest accuracy among all printers tested. The region had a significant effect on the deviation measured, with the abutment body showing the least deviation. Among the 3D printers evaluated, A2D4K by HeyGears and Carbon M2 printers demonstrated the highest accuracy in terms of both precision and trueness.

The Effect of Different Storage Conditions and Scan Delays on the Mean Gray Value (MGV) on Photostimulable Storage Phosphor (PSP) Plate Images

Amaç: Bu çalışmanın amacı, ışıkla uyarılabilen fosfor (Photostimulable Storage Phosphor, PSP) plakalarının ışınlama-tarama süreçleri arasındaki farklı tarama gecikmeleri (bekletme süresi) ve saklama koşullarının görüntülerindeki Ortalama Gri Değer (OGD) değişimine etkisini değerlendirmekti. Gereç ve Yöntem: Saklama koşuluna göre oluşturulan dört grupta [Tek Kılıf Aydınlık (TKA), Tek Kılıf Karanlık (TKK), Tek Kılıf Karton Aydınlık (TKKA), Tek Kılıf Karton Karanlık (TKKK)] farklı bekletme sürelerinde toplam 40 görüntü elde edildi. ImageJ (National Institutes of Health, Bethesda, MD) programı ile görüntülerin OGD’leri ölçüldü. Saklama koşullarını karşılaştırmak için tek yönlü, bekletme sürelerini karşılaştırmak için iki yönlü tekrarlı ölçümlü ANOVA testleri kullanıldı. Her bir gruptaki bekletme süreleri ile 0 dk. (altın standart) OGD’lerin karşılaştırılması için eşleştirilmiş örneklemler t-testi uygulandı. p<0.05 istatistiksel olarak anlamlı kabul edildi. BULGULAR: On dk. ile altın standart arasındaki OGD farkı sadece TKA grubunda istatistiksel olarak anlamlıydı (p<0.05). Herhangi bir bekletme süresinde, saklama koşulları OGD’leri arasında istatistiksel olarak anlamlı fark yoktu (p>0.05). Altın standart dışındaki diğer bekletme süreleri karşılaştırıldığında, 24 saat bekletme süresi ile diğer bekletme süreleri OGD’leri arasında istatistiksel olarak anlamlı fark vardı (p<0.05). Sonuç: Plakların ışınlama sonrası karanlık ortamda ve karton koruyucu içinde 10 dk.’yı geçmeyen bekletme süresi ile bekletilmesinin OGD’ye etkisi önemsizdi. Sadece plastik bariyerde ve 24 saat sürede bekletilen plakların görüntülerdeki OGD artışı dikkat çekiciydi.

Screening of volatile organic compounds emitted from different packaging materials: case study on fresh-cut artichokes

In the present work, the emission of volatile organic compounds (VOCs) from plastic packaging materials and their migration to the packaged product was investigated. Fresh-cut artichokes were chosen as a case study owing to their nutritional profile, including phenolic compounds, antioxidants, inulin and fiber, and the high market demand. Artichokes were cut into quarters, packaged in active modified atmosphere (5% O2 and 10% CO2) in three different micro-perforated (MP) packages (polypropylene (PP), polypropylene/polyamide (PP/PA) and polylactic acid (PLA)) and stored for 10 days at 5° C. For the detection of VOCs on the packaging materials and in the packaged product, three approaches were considered. First, VOCs emitted from a piece of package were extracted by solid phase micro extraction (SPME), at 30°C, and analyzed by gas-chromatography coupled to mass spectrometry (GC–MS). Second, VOCs from the headspace of the empty closed bag were extracted by SPME, at 5°C, to understand if any packaging volatile compound was transferred into the package atmosphere in this temperature condition. Finally, packaged artichokes were also analyzed by SPME/GC–MS to detect any presence of packaging volatiles compared to the fresh product. Emitted VOCs were different according to the material; in particular PP/PA emitted the greatest number of VOCs, most of them belonging to the class of branched alkanes and alkenes, such as 4-methyl-heptane, 2,4-dimethyl-heptane, 4-methyl-octane and 2,4 dimethyl heptene; PP emitted octane, aldehydes, and 1-metoxy-2-propanol; PLA emitted aldehydes and propanoic acid. Some VOCs were found into the atmosphere of the empty bags as well as on packaged artichokes, while they were not found in the fresh samples before packaging, suggesting that a study on the emission of VOCs from different plastic materials in contact with food and on the effect of different storage conditions is very critical for a better understanding of this issue.

The Effect of Different Storage Conditions on the Levels of Bisphenol A in Bottled Drinking Water in Jeddah City, Saudi Arabia

Bisphenol A (BPA) in drinking water sources is a significant concern in society because BPA is one of the endocrine disruption compounds (EDCs) that can cause hazards to human health even at extremely low concentration levels. This study investigated the leaching potential of BPA from drinking water bottles in five brands of bottled drinking water in Jeddah, Saudi Arabia, using high-performance liquid chromatography with a diode array detector (HPLC-DAD). To the best of our knowledge, this is the first study to evaluate the level of BPA in bottled water in the city of Jeddah. The separation was carried out under isocratic elution, and the detector was set to UV mode. Low levels of BPA were detected in all samples from polyethylene terephthalate (PET) containers. The mean concentration of BPA in water bottles stored at room temperature for 30 days was 9.46 ng·L−1, while the concentration of BPA in water bottles exposed to sunlight and boiling water bath was 16.13 ng·L−1 and 14.7 ng·L−1, respectively. Although the results show that the daily consumed concentration of BPA for an adult with 60 kg of body weight is 32.26 ng, which is lower than the total tolerable daily intake limit of BPA, health risks from the consumption of bottled water may increase after UV exposure for an extended time.

Effects of time and temperature of storage on chemical and nutritional characteristics of raw milk for Provolone Valpadana PDO cheesemaking: a multivariate approach.

We evaluated the possibility of increasing the storage temperature of raw milk for Provolone Valpadana cheesemaking, to identify the most suitable conditions of time and temperature for a pre-maturation process. We used Principal Component Analysis (PCA) to analyze the overall effects of different storage conditions on chemical, nutritional and technological characteristics of the raw milk. Four different thermal storage cycles, two at fixed temperature/time (6 and 12°C for 60 h) and two with two-phase thermal cycle (10 and 12°C for 15 h, followed by refrigeration at 4°C for 45 h) were studied. Although a moderate heterogeneity among raw milks from the 11 producers of Provolone Valpadana cheese was observed, PCA revealed the critical aspects of the extreme storage conditions (60 h of refrigeration). Some samples resulted in anomalous behaviors, probably related to unexpected fermentation phenomena occurring with increasing storage temperature. The acidification and the increase in the contents of lactic acid, soluble calcium, and degree of retinol isomerization observed in the anomalous samples can compromise the technological functionality of milk. Conversely, the storage with a two-phase thermal cycle did not lead to variations in any measured characteristic, suggesting that mild refrigeration conditions (10 or 12°C for 15 h followed by 4°C for 45 h) could be a good compromise in favoring milk pre-maturation without altering its quality characteristics.

Effects of storage conditions on the stability of blood-based markers for the diagnosis of Alzheimer's disease.

Alzheimer's disease (AD) is considered the most common cause of dementia in older people. Recently, blood-based markers (BBM) Aβ1-42, Aβ1-40, and phospho Tau181 (p-Tau181) have demonstrated the potential to transform the diagnosis and prognostic assessment of AD. Our aim was to investigate the effect of different storage conditions on the quantification of these BBM and to evaluate the interchangeability of plasma and serum samples. Forty-two individuals with some degree of cognitive impairment were studied. Thirty further patients were retrospectively selected. Aβ1-42, Aβ1-40, and p-Tau181 were quantified using the LUMIPULSE-G600II automated platform. To assess interchangeability between conditions, correction factors for magnitudes that showed strong correlations were calculated, followed by classification consistency studies. Storing samples at 4 °C for 8-9days was associated with a decrease in Aβ fractions but not when stored for 1-2days. Using the ratio partially attenuated the pre-analytical effects. For p-Tau181, samples stored at 4 °C presented lower concentrations, whereas frozen samples presented higher ones. Concerning classification consistency in comparisons that revealed strong correlations (p-Tau181), the percentage of total agreement was greater than 90 % in a large number of the tested cut-offs values. Our findings provide relevant information for the standardization of sample collection and storage in the analysis of AD BBM in an automated platform. This knowledge is crucial to ensure their introduction into clinical settings.

Exploring the Effect of Different Storage Conditions on the Aroma Profile of Bread by Using Arrow-SPME GC-MS and Chemometrics.

In the present feasibility study, SPME Arrow-GC-MS method coupled with chemometric techniques, was used for investigating the impact of two different storage conditions, namely freezing and refrigeration, on volatile organic compounds (VOCs) of different commercial breads. The SPME Arrow technology was used as it is a novel extraction technique, able to address issues arising with traditional SPME fibers. Furthermore, the raw chromatographic signals were analysed by means of a PARAFAC2-based deconvolution and identification system (PARADISe approach). The use of PARADISe approach allowed for an efficient and rapid putative identification of 38 volatile organic compounds, including alcohols, esters, carboxylic acids, ketones, and aldehydes. Additionally, Principal Component Analysis, applied on the areas of the resolved compounds, was used to investigate the effects of storage conditions on the aroma profile of bread. The results revealed that the VOC profile of fresh bread is more similar to the one of bread stored in the fridge. Furthermore, there was a clear loss of aroma intensity in frozen samples, which could be explained by phenomena related to different starch retrogradation that occurs during freezing and refrigeration. However, considering the limited number of investigated samples, this study must be considered as a proof of concept; a more statistically representative sampling and further examinations of other properties, such as bread texture, need to be performed to better understand whether samples destined for eventual analysis should be frozen or refrigerated.

Effects of Different Storage Conditions on Lipid Stability in Mice Tissue Homogenates.

Lipids are biomolecules involved in numerous (patho-)physiological processes and their elucidation in tissue samples is of particular interest. However, tissue analysis goes hand in hand with many challenges and the influence of pre-analytical factors can intensively change lipid concentrations ex vivo, compromising the results of the whole research project. Here, we study the influence of pre-analytical factors on lipid profiles during the processing of homogenized tissues. Homogenates from four different mice tissues (liver, kidney, heart, spleen) were stored at room temperature as well as in ice water for up to 120 min and analyzed via ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS). Lipid class ratios were calculated since their suitability as indicators for sample stability has been previously illustrated. Only approx. 40% of lipid class ratios were unchanged after 35 min, which was further reduced to 25% after 120 min during storage at room temperature. In contrast, lipids in tissue homogenates were generally stable when samples were kept in ice water, as more than 90% of investigated lipid class ratios remained unchanged after 35 min. Ultimately, swift processing of tissue homogenates under cooled conditions represents a viable option for lipid analysis and pre-analytical factors require more attention to achieve reliable results.

Optimization of electrolysis process, storage conditions and sterilization effect of slightly acidic electrolytic water prepared by titanium suboxide electrode

Slightly acidic electrolyzed (SAEW) is a new electrochemical fungicide containing a certain amount of active chlorine and active oxygen. SAEW has the characteristics of broad spectrum, harmless and high efficiency of sterilization. At present, there is little systematic research on the preparation, storage and sterilization of SAEW. However, the anode material, preparation parameters and storage conditions are crucial to the performance indicators and sterilization effect of SAEW. In this paper, titanium suboxide electrode with better safety was used as anode. Firstly, we investigated the effects of various preparation parameters (NaCl concentration, NaCl and HCl mixture concentration and current density) on the preparation of SAEW. The optimized preparation parameters were 1.0 g L−1 of NaCl and HCl mixed to an initial pH of 2.7 and a current density of 15 mA cm−2. Next, the effects of different storage conditions (open-dark, closed-dark, closed-light, open-light) on its indexes were investigated. It was confirmed that the optimal storage condition was closed-dark. Finally, the sterilization effect on Escherichia coli, Staphylococcus aureus and Canidia albicans was investigated. The best sterilization effect was E. coli, and the sterilization effect of all three bacteria gradually increased with the extension of contact time. The above study shows that SAEW prepared by titanium suboxide electrode has good performance in terms of performance indexes and sterilization effect, and has the prospect of practicality.

Effects of different storage conditions on microbial community and quality changes of greater amberjack (Seriola dumerili) fillets

To improve the safety and extend the shelf life of fish fillets, four different temperatures were used to preserve the greater amberjack. The microbial diversity and quality changes were determined. The freezing point of greater amberjack fillets was determined by analyzing the freezing curve, and four storage temperatures were applied, room temperature (20 °C), refrigeration (4 °C), ice temperature (-1 °C) and partial freezing temperature (-3 °C). The shelf life of greater amberjack fillets stored at 20 °C, 4 °C, -1 °C and -3 °C was 30 h, 7 d, 21 d and 36 d, respectively based on TVC and TVB-N results. The microbial community indicated the bacterial richness and diversity were higher at the end of the storage time under -1 °C and -3 °C. Pseudomonas was the specific spoilage bacteria of greater amberjack stored at low temperature. The growth of spoilage bacteria was effectively inhibited when the sample was stored at -1 °C and -3 °C, which were more conducive to maintaining the initial bacterial phase, delaying the spoilage, and prolonging the shelf life. This study showed promising storage temperatures of -1 °C and -3 °C for fish fillets, which would prolong the shelf life and improve the safety of preserved samples.

The Effect of Different Storage Conditions on Phytochemical Composition, Shelf-Life, and Bioactive Compounds of Voghiera Garlic PDO.

Voghiera garlic is an Italian white garlic variety which obtained in 2010 the Protected Designation of Origin. It is widely used for culinary purposes or as an ingredient for supplement production due to its phytochemical compositions. The storage conditions seem to be crucial to retain the high quality of garlic bulbs and their by-products, taking into account the high importance of organosulfur and phenolic compounds for the bioactive potency of garlic and its shelf-life. This study aims to examine the effect of storage on the phytochemical composition, biological effects, and shelf-life of Voghiera garlic PDO. In detail, we considered (i) -4 °C (industrial storage) for 3, 6, and 9 months; (ii) +4 °C for 3 months (home conservation), and (iii) -4 °C for 3 months, plus +4 °C for another 3 months. We focused our attention on the organosulfur compounds, total condensed tannins, flavonoids, phenolic compounds, and related antioxidant activity changes during the storage period. To evaluate the bioactive effects, the Voghiera garlic extracts at different storage conditions were administered to a breast cancer cell line, while antioxidant and anti-inflammatory activity was detected using macrophage RAW 264.7 cells. We observed a decrease in sulfur compounds after 6 months which correlated to a decrease in bioactive effects, while the number of antioxidant compounds was stable during the storage period, showing the good effect of refrigerated temperature in maintaining garlic bulb shelf-life.

Evaluation of Germination under Different Storage Conditions of Four Endemic Plant Species from Ethiopia: Implications for Ex Situ Conservation in Seed Banks

The conservation of endemic plant species is a major concern, as the species are with restricted distribution range. Since in situ conservation alone will not guarantee their maintenance, ex situ conservation measures must be undertaken to support the conservation of these species. Investigation of the impact of the storage environment of seeds gives baseline information. Therefore, this study was conducted to investigate the effect of different storage conditions (room temperature, 4 °C and −10 °C) and different storage periods over one year. Four Ethiopian endemic plant species, namely Euryops pinifolius, Kniphofia foliosa, Lobelia rhynchopetalum, and Solanecio gigas, were considered. Multivariate analyses revealed a significant (p < 0.05) effect of storage condition and period on seed germination. The storage of seeds at room temperature before drying may not be recommended for short-term storage of the studied species, except K. foliosa. After drying of seeds, E. pinifolius, K. foliosa, and L. rhynchopetalum seeds showed high germination percentage (above 80%) after storage even at sub-zero temperatures for one year. The germination percentage of S. gigas stored at room temperature before drying for thirteen months was 60% and that stored at 4 °C was 6%, which indicates that the seeds may be categorized under intermediate storage behavior. The three species can be grouped in the orthodox seed storage category. Research on desiccation screening should be undertaken to predict an optimal combination of storage moisture content and suitable storage temperature to determine storage category with certainty.

Effect of different storage conditions on the stability and safety of almonds.

Almond production in Portugal is of great importance for the economy of their main producing areas. However, the contamination of these nut fruits with fungi and mycotoxins poses a significant risk to food safety and security. This work intended to evaluate the influence of storage conditions on the microbial and mycotoxin stability and safety of almonds throughout long-term storage. Two almond varieties-Lauranne and Guara-were submitted to three different storage conditions, namely, 4°C with noncontrolled relative humidity (RH), 60% RH at 25°C, and 70% RH at 25°C, for a storage period of 9 months. Samples were collected after 0, 3, 6, and 9 months of storage and analyzed for microbial loads (aerobic mesophiles, yeasts, and molds), mold incidence and diversity, and mycotoxin contamination. In total, 26 species were identified belonging to 6 genera: Aspergillus, Cladosporium, Fusarium, Penicillium, Paecilomyces, and Talaromyces. For the variety Guara, mycotoxins related to Aspergillus sect. Flavi, such as aflatoxins, averufin, versicolorin C, and norsolorinic acid, were detected only after 9 months of storage at 70% and 60% RH. Penicillium mycotoxins, such as quinolactacin A and roquefortine C, were also detected. For the variety Lauranne, Penicillium mycotoxins were detected, such as citrinin, quinolactacins A and B, roquefortines C and D, cyclopenin, cyclopenol, penitrem A, viridicatin, and viridicatol. Mycotoxins related to Aspergillus, such as aspulvinone E, flavoglaucin, paspalin, asperglaucide, asperphenamate, cyclo(L-Pro-L-Tyr), and cyclo(L-Pro-L-Val), were also detected. PRACTICAL APPLICATION: (Optional, for JFS Research Articles ONLY) The quality of almonds depends on the storage period and the RH and temperature at which they are stored. Storage of almonds at 60% RH at 25°C is a good storage condition to maintain the stability and safety of nuts in terms of microbial and mycotoxin contaminations.

Physicochemical and functional properties of egg white peptide powders under different storage conditions.

This study aims to evaluate the effects of different storage conditions (temperature and relative humidity) on the physicochemical and functional properties of egg white peptide powders (EWPPs). The samples (EWPPs) were stored for 28 d under four conditions (4°C, 50% RH; 4°C, 75% RH; 25°C, 50% RH; 25°C, 75% RH). Results showed that storage temperature and relative humidity had a significant effect on the physicochemical and functional properties of EWPPs. The contents of antioxidant amino acids such as histidine, tyrosine, tryptophan, and lysine were reduced significantly under different storage conditions, which resulted in the decrease of the antioxidant activity of EWPPs. Circular dichroism spectroscopy analysis indicated that the secondary structure of EWPPs changed from the regular structure to the irregular coiled structure during the storage. Additionally, the hydrophobic groups of the EWPPs originally embedded inside the molecules were exposed to the surface of the molecules during the storage, which led to an aggregation of EWPPs molecule and a decrease in solubility of EWPPs. The aggregation of EWPPs molecules resulted in a decrease in emulsification, emulsification stability, foaming ability and foaming stability of the EWPPs. Therefore, different storage conditions do have an impact on the physicochemical and functional properties of EWPPs. Lower temperature and humidity storing conditions were beneficial to retain the functional property of the EWPPs.

Effect of different storage conditions on the quality attributes of sweet lime juice subjected to pulsed light and thermal pasteurization

Overview of all the experimental conditions and responses for the shelf-life study of sweet lime juice.

Effects of different storage conditions on the colour, antioxidant activity, and volatile components of edible roses

This study examined the changes in petal colour, antioxidant activity, and volatile components of miniature roses, one of the most popular edible flowers in Japan, when stored under different conditions. In total, six storage treatments were examined: 5°C and 75% relative humidity (RH) for 3 d (75%-3D plot); 5°C and 75% RH for 6 d (75%-6D plot); 5°C and 96% RH for 3 d (96%-3D plot); 5°C and 96% RH for 6 d (96%-6D plot); -25°C for 3 d (F-3D plot); and -25°C for 6 d (F-6D plot). As a control, a measurement was conducted on day 0 without storage (0D plot). There were no significant differences in flower width, fresh weight, and total polyphenol concentrations among the storage treatments. DPPH radical scavenging activity decreased during storage, especially during freezing storage, but was higher in the 75%-3D plot than that in the other storage conditions. A colour difference was found between the cold and freezing storage treatments because of colour changes after freezing and thawing. The sweet aroma decreased during storage, and a bad smell was detected in the freezing treatment. Freezing storage was thus unsuitable for storing edible roses because it degraded colour, decreased antioxidant concentrations, and increased a bad smell. The flower colour measured using a chromameter and DPPH radical scavenging activity in the 75%-3D plot were not significantly different from those in the 0D plot. Further, the total volatile component contents in all 6D plots were lower than those in all the 3D plots during cold storage. Therefore, these findings indicate that edible roses should be stored under refrigeration at 75% RH and used within approximately three days after purchase. This information can be helpful to food, cosmetic, and pharmaceutical industries that require preservation of edible flowers.